Detailed view for TcCLB.506743.170

Basic information

TDR Targets ID: 45137
Trypanosoma cruzi, Met-10+ like-protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8743 | Length (AA): 508 | MW (Da): 56666 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02475   Met-10+ like-protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0009019   tRNA (guanine-N1-)-methyltransferase activity  
GO:0030488   tRNA methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
165 434 2frn (A) 19 267 21.00 0.0000000000063 0.96 0.59 0.48
290 406 1xxl (A) 8 115 10.00 0.0000002 0.05 0.35 -1.04
114 486 5hjm (A) 26 331 22.00 0.0000000011 1 0.720352 0.93
155 436 3k6r (A) 9 269 20.00 0 1 0.588418 0.68
258 357 3axs (A) 1 118 26.00 0 0.87 0.37315 -0.38
270 400 3gdh (A) 662 769 34.00 0.13 0.32 0.253974 0.76
277 357 1nv8 (A) 104 184 22.00 0 0.22 0.337749 0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127369)

Species Accession Gene Product
Arabidopsis thaliana AT4G27340   Met-10+ like family protein
Arabidopsis thaliana AT3G56120   tRNA (guanine(37)-N1)-methyltransferase 1
Babesia bovis BBOV_III001260   Met-10+ like-protein
Brugia malayi Bm1_47670   Met-10+ like-protein
Candida albicans CaO19.1305   similar to S. cerevisiae YHR070W
Candida albicans CaO19.8885   similar to S. cerevisiae YHR070W
Caenorhabditis elegans CELE_C53A5.2   Protein C53A5.2
Caenorhabditis elegans CELE_C53A5.16   Protein C53A5.16, isoform A
Caenorhabditis elegans CELE_C53A5.17   Protein C53A5.17
Cryptosporidium hominis Chro.60592   hypothetical protein
Cryptosporidium parvum cgd6_5130   Trm5 RNA methyltransferase
Dictyostelium discoideum DDB_G0279739   tRNA -methyltransferase
Drosophila melanogaster Dmel_CG32281   CG32281 gene product from transcript CG32281-RA
Echinococcus granulosus EgrG_000513700   protein of unknown function Met10
Entamoeba histolytica EHI_111740   Met-10 -like protein
Echinococcus multilocularis EmuJ_000513700   protein of unknown function Met10
Giardia lamblia GL50803_4164   Met-10+ protein
Homo sapiens ENSG00000126814   tRNA methyltransferase 5
Leishmania braziliensis LbrM.16.0320   hypothetical protein, conserved
Leishmania donovani LdBPK_160320.1   Met-10+ like-protein, putative
Leishmania infantum LinJ.16.0320   hypothetical protein, conserved
Leishmania major LmjF.16.0310   hypothetical protein, conserved
Leishmania mexicana LmxM.16.0310   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05906   hypothetical protein
Mus musculus ENSMUSG00000034442   TRM5 tRNA methyltransferase 5
Neospora caninum NCLIV_018040   hypothetical protein
Oryza sativa 9271157   Os02g0606301
Oryza sativa 9270169   Os01g0390400
Onchocerca volvulus OVOC7293  
Plasmodium berghei PBANKA_0815300   met-10+ like protein, putative
Plasmodium falciparum PF3D7_0914300   met-10+ like protein, putative
Plasmodium knowlesi PKNH_0712200   met-10+ like protein, putative
Plasmodium yoelii PY04160   unknown protein
Saccharomyces cerevisiae YHR070W   Trm5p
Schistosoma japonicum Sjp_0086490   ko:K07055 TRM5 tRNA methyltransferase 5 homolog, putative
Schistosoma mansoni Smp_045760   hypothetical protein
Schmidtea mediterranea mk4.002048.01   tRNA
Schmidtea mediterranea mk4.006682.01   tRNA
Schmidtea mediterranea mk4.006682.00  
Trypanosoma brucei gambiense Tbg972.8.5720   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.5720   tRNA (guanine(37)-N1)-methyltransferase
Trypanosoma congolense TcIL3000_8_5510   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506743.170   Met-10+ like-protein, putative
Trypanosoma cruzi TcCLB.503473.20   Met-10+ like-protein, putative
Toxoplasma gondii TGME49_243280   Met-10+ like-protein
Theileria parva TP03_0709   hypothetical protein
Trichomonas vaginalis TVAG_038970   conserved hypothetical protein

Essentiality

TcCLB.506743.170 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5720 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5720 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5720 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5720 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YHR070W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0815300 Plasmodium berghei Essential plasmo
TGME49_243280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.506743.170 (Trypanosoma cruzi), Met-10+ like-protein, putative
Title for this comment
Comment